Open AccessIsolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro.
Author(s) -
Henry Furneaux,
K K Perkins,
G A Freyer,
Jesús Arenas,
J Hurwitz
Publication year - 1985
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.13.4351
Subject(s) - micrococcal nuclease , hela , rna splicing , in vitro , nucleic acid , globin , rna , nuclease , microbiology and biotechnology , biochemistry , messenger rna , biology , chemistry , dna , histone , gene , nucleosome
A nuclear extract from HeLa cells has been separated by DEAE-cellulose chromatography into two fractions, both of which are required for mRNA splicing in vitro. Both fractions are heat labile and sensitive to N-ethylmaleimide. The activity of one of the fractions was abolished by preincubation with micrococcal nuclease, while the other fraction was unaffected by this treatment. This abolition indicates an essential nucleic acid component. Fractions I and II are required for the in vitro splicing of human beta-globin and adenovirus transcripts.
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