Open AccessOpposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene.
Author(s) -
Peggy J. Farnham,
John Abrams,
Robert T. Schimke
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.12.3978
Subject(s) - dihydrofolate reductase , transcription (linguistics) , biology , gene , microbiology and biotechnology , rna , nucleotide , genetics , linguistics , philosophy
We have characterized a transcription unit in the 5' flanking region of the mouse gene encoding dihydrofolate reductase (EC 1.5.1.3) that is oriented in the opposite direction to that of dihydrofolate reductase transcription. These opposite-strand RNAs are 180-240 nucleotides long, differing in length at their 5' ends. They are abundant in nuclear RNA and are not polyadenylylated. We suggest that the promoter region of the dihydrofolate reductase gene functions in a bidirectional manner to produce both these RNAs and dihydrofolate reductase mRNAs.