Open AccessDNA sequence and characterization of human class II major histocompatibility complex beta chains from the DR1 haplotype.
Author(s) -
John I. Bell,
Pila Estess,
Tom St. John,
Randall K. Saiki,
Diane Watling,
Henry A. Erlich,
Hugh O. McDevitt
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.10.3405
Subject(s) - biology , beta (programming language) , microbiology and biotechnology , haplotype , human leukocyte antigen , major histocompatibility complex , genetics , peptide sequence , clone (java method) , sequence analysis , gene , antigen , genotype , computer science , programming language
Two HLA class II beta-chain clones from a cell line homozygous for the DR1 haplotype have been characterized and sequenced. They represent a DR beta chain (2918.4) and a DQ beta chain (2918.8). Clone 2918.4 has been used to select mRNA from a lymphoblastoid cell line, and this was injected into Xenopus oocytes with mRNA selected with a DR alpha chain. The translation products were immunoprecipitated with a beta-chain-specific monoclonal antibody and electrophoresed on two-dimensional gels. This revealed positive signals in the positions predicted for beta and alpha chains. Sequence comparisons of 2918.4 with previously published DR beta-chain sequences confirm the presence of two regions of variability in the membrane distal domain. Analysis of the sequence of 2918.8 identified it as a DQ beta chain identical to one previously published from a DR3,w6 cell line. We speculate, therefore, that the DQ beta sequence represents the DQ1 specificity shared by the DR1 and DRw6 haplotypes.