Open AccessDevelopmentally regulated expression of a chicken muscle-specific gene in stably transfected rat myogenic cells.
Author(s) -
Uri Nudel,
David Greenberg,
Charles P. Ordahl,
Ora Saxel,
Sara Neuman,
David Yaffe
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.10.3106
Subject(s) - transfection , biology , microbiology and biotechnology , gene , gene expression , skeletal muscle , myocyte , actin , cellular differentiation , messenger rna , recombinant dna , regulation of gene expression , genetics , endocrinology
To test the evolutionary conservation of DNA sequences specifying the developmentally regulated expression of the skeletal muscle actin gene, a recombinant plasmid containing the chicken skeletal muscle actin gene was introduced into rat myogenic cells. In a significant number of isolated clones, the accumulation of chicken actin mRNA increased greatly during differentiation. To test the expression in myogenic cells of a gene that is normally expressed during terminal differentiation of another tissue, rat myogenic cells were transfected with a mouse/human beta-globin chimeric gene. A decrease by a factor of 2-3 in the amount of globin mRNA during differentiation was observed in most clones in which the gene was expressed. The results indicate the conservation of the muscle-specific regulatory DNA sequences for more than 300 Myr.