Gene encoding human growth hormone-releasing factor precursor: structure, sequence, and chromosomal assignment. | Zendy

Kelly E. Mayo | Zendy; Gail Cerelli | Zendy; R V Lebo | Zendy; Barry D. Bruce | Zendy; Michael G. Rosenfeld | Zendy; Ronald M. Evans | Zendy

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Gene encoding human growth hormone-releasing factor precursor: structure, sequence, and chromosomal assignment.

Author(s) -

Kelly E. Mayo,

Gail Cerelli,

R V Lebo,

Barry D. Bruce,

Michael G. Rosenfeld,

Ronald M. Evans

Publication year - 1985

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.82.1.63

Subject(s) - cosmid , exon , biology , intron , gene , genomic dna , genetics , microbiology and biotechnology , southern blot , genomic library , peptide sequence

We have isolated and characterized overlapping clones from phage lambda and cosmid human genomic libraries that predict the entire structure of the gene encoding the precursor to human growth hormone-releasing factor. The gene includes five exons spanning 10 kilobase pairs of human genomic DNA. There appears to be a segregation of distinct functional regions of the GRF precursor and its mRNA into the five exons of the gene. The DNA sequences of all exons, intron/exon boundaries, and 5' and 3' flanking regions are presented. Dot-blot analysis of DNA from high resolution dual-laser-sorted human chromosomes indicates that the single-copy growth hormone-releasing factor gene is located on human chromosome 20.

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