Open AccessDuplicated heavy metal control sequences of the mouse metallothionein-I gene.
Author(s) -
Anthony D. Carter,
Barbara K. Felber,
Maryjane Walling,
Marie-France Jubier,
Carl J. Schmidt,
Dean H. Hamer
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.23.7392
Subject(s) - gene duplication , biology , gene , metallothionein , plasmid , genetics , upstream activating sequence , dna , regulatory sequence , point mutation , transcription (linguistics) , microbiology and biotechnology , regulation of gene expression , mutation , gene expression , promoter , philosophy , linguistics
We present evidence that two distinct regions of the DNA upstream from the mouse metallothionein-I gene contain metal-responsive regulatory sites. This result was obtained by analyzing a systematic series of deletion, insertion, duplication, and clustered point mutations introduced into cultured cells on a simian virus 40 plasmid vector. The two upstream regions contain a duplicated evolutionarily conserved DNA sequence. While either upstream region is sufficient to confer heavy metal responsiveness, both are required to give maximal levels of induced transcription.