Open AccessSequence of the bacteriophage SP01 gene coding for transcription factor 1, a viral homologue of the bacterial type II DNA-binding proteins.
Author(s) -
Jonathan Greene,
S. Brennan,
Deborah J. Andrew,
Catherinec . Thompson,
S H Richards,
Robertl . Heinrikson,
E. Peter Geiduschek
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.22.7031
Subject(s) - biology , dna , gene , dna binding protein , dna binding site , microbiology and biotechnology , transcription (linguistics) , bacteriophage , bacillus subtilis , genetics , single stranded binding protein , hmg box , transcription factor , promoter , escherichia coli , gene expression , bacteria , linguistics , philosophy
The Bacillus subtilis phage SP01, whose DNA contains 5-hydroxymethyluracil (hmUra) in place of thymine, codes for an abundant, small, basic protein called TF1. TF1 binds preferentially to hydroxymethyluracil-containing DNA and thereby selectively inhibits transcription of such DNA in vitro. The gene for TF1 has been sequenced. We find that this viral protein is a homologue of the ubiquitous bacterial type II DNA-binding proteins. The three-dimensional structure of one of these bacterial proteins has recently been determined. We are able to discern common as well as distinctive features in the amino acid sequence and the three-dimensional structure of the homologous viral protein.