Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome. | Zendy

Gesine Fleischmann | Zendy; Gert O. Pflugfelder | Zendy; E K Steiner | Zendy; Kashayar Javaherian | Zendy; Gary C. Howard | Zendy; J C Wang | Zendy; Sarah C. R. Elgin | Zendy

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Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome.

Author(s) -

Gesine Fleischmann,

Gert O. Pflugfelder,

E K Steiner,

Kashayar Javaherian,

Gary C. Howard,

J C Wang,

Sarah C. R. Elgin

Publication year - 1984

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.81.22.6958

Subject(s) - topoisomerase , biology , nucleolus , transcription (linguistics) , microbiology and biotechnology , polymerase , dna , rna polymerase , polytene chromosome , rna polymerase i , rna polymerase ii , rna , genetics , drosophila melanogaster , gene , rna dependent rna polymerase , gene expression , promoter , linguistics , philosophy , cytoplasm

The distribution of DNA topoisomerase I within Drosophila polytene chromosomes was observed by immunofluorescent staining with affinity-purified antibodies. The enzyme is preferentially associated with active loci, as shown by prominent staining of puffs. The heat shock loci 87A-87C are stained after, but not before, heat shock induction. A detailed comparison of the distribution of topoisomerase I with that of RNA polymerase II reveals a similar, although not identical, pattern of association. Topoisomerase I is also found in association with the nucleolus, the site of transcription by RNA polymerase I.

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