Open Access(Pro)insulin associates with Golgi membranes of pancreatic B cells.
Author(s) -
Lelio Orci,
Mariella Ravazzola,
Alain Perrelet
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.21.6743
Subject(s) - golgi apparatus , endoplasmic reticulum , immunolabeling , secretory pathway , granule (geology) , microbiology and biotechnology , membrane , biology , intracellular , insulin , secretory protein , biochemistry , chemistry , secretion , endocrinology , immunohistochemistry , immunology , paleontology
The immunocytochemical demonstration of (pro)insulin on intracellular membrane compartments of the pancreatic B cell reveals that the immunolabeling detected by the protein A/gold method is associated, at the level of the Golgi apparatus, with the inner aspect of the cisternal membranes; on the secretory granules, by contrast, insulin immunoreactive sites predominate over the granule core, and very little immunoreactivity is associated with the granule membrane. The localization of (pro)insulin immunoreactivity on Golgi membranes is compatible with the presence, at this level, of specific binding sites for (pro)insulin, which could be related to the proteolytic processing or sorting out (or both) of these peptides on their way from the rough endoplasmic reticulum to the storage secretory granules.