tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli. | Zendy

Shunsuke Ishii | Zendy; Kazuyuki Kuroki | Zendy; Fumio Imamoto | Zendy

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tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli.

Author(s) -

Shunsuke Ishii,

Kazuyuki Kuroki,

Fumio Imamoto

Publication year - 1984

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.81.2.409

Subject(s) - operon , biology , gene , escherichia coli , transcription (linguistics) , rna polymerase , genetics , nucleic acid sequence , microbiology and biotechnology , linguistics , philosophy

The promoter-proximal portion of the operon containing the Escherichia coli nusA gene has been cloned. Its nucleotide sequence shows that genes for tRNAMetf2 and a 15-kilodalton protein of unknown function precede the nusA protein gene. The sequence suggests that the three genes form a single transcription unit. Consistent with this hypothesis, purified RNA polymerase formed full-length transcripts on the cloned DNA in vitro, although transcription was frequently arrested at the intercistronic site(s) between the gene for tRNAMetf2 and the 15-kilodalton protein.

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