Open AccesstRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli.
Author(s) -
Shunsuke Ishii,
Kazuhiko Kuroki,
Fumio Imamoto
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.2.409
Subject(s) - operon , biology , gene , escherichia coli , transcription (linguistics) , rna polymerase , genetics , nucleic acid sequence , microbiology and biotechnology , linguistics , philosophy
The promoter-proximal portion of the operon containing the Escherichia coli nusA gene has been cloned. Its nucleotide sequence shows that genes for tRNAMetf2 and a 15-kilodalton protein of unknown function precede the nusA protein gene. The sequence suggests that the three genes form a single transcription unit. Consistent with this hypothesis, purified RNA polymerase formed full-length transcripts on the cloned DNA in vitro, although transcription was frequently arrested at the intercistronic site(s) between the gene for tRNAMetf2 and the 15-kilodalton protein.