Open AccessChloroplast tRNA Gly gene contains a long intron in the D stem: Nucleotide sequences of tobacco chloroplast genes for tRNA Gly (UCC) and tRNA Arg (UCU)
Author(s) -
Hiroshi Deno,
Masahiro Sugiura
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.2.405
Subject(s) - transfer rna , intron , gene , biology , base pair , genetics , chloroplast dna , rna , microbiology and biotechnology , chloroplast
The nucleotide sequences of tobacco chloroplast genes for tRNAGly (UCC) and tRNAArg (UCU) have been determined. The tRNAGly gene has a 691-base-pair intron located in the D stem while the tRNAArg gene does not have any intron. The tRNAGly and tRNAArg genes are encoded on the same strand and separated by a 169-base-pair spacer. The tRNAGly gene is transcribed as a 900-base precursor RNA molecule. The tRNAGly and tRNAArg deduced from the DNA sequences show 84% and 55% sequence homologies withEscherichia coli tRNAGly (UCC) and phage T4 tRNAArg (UCU), respectively.