Open AccessMagnetic susceptibility of oxy- and carbonmonoxyhemoglobins.
Author(s) -
Joseph P. Savicki,
Georgina Lang,
Masao IkedaSaito
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.17.5417
Subject(s) - magnetic susceptibility , paramagnetism , magnetometer , chemistry , analytical chemistry (journal) , magnetic field , nuclear magnetic resonance , mössbauer spectroscopy , spectroscopy , condensed matter physics , crystallography , physics , chromatography , quantum mechanics
The room temperature magnetic susceptibilities of human and carp oxy- and carbonmonoxyhemoglobin solutions were measured in a 30-gauss (1 G = 10(-4) T) field with a superconducting magnetometer. To within experimental uncertainty, the susceptibility was the same for both the oxy and carbonmonoxy forms, and salt concentration did not effect it. A variety of sample preparations were used; the iron chemical state was verified by Mössbauer spectroscopy. A value of -0.580 +/- 0.010 X 10(-6) centimeter-gram-second (cgs) system was obtained for the mass susceptibility of the protein. We attribute the paramagnetism sometimes observed in oxyhemoglobin solutions to the presence of a small amount of the deoxy form.