Open AccessIdentification of a cloned cytokinin biosynthetic gene
Author(s) -
Gerard F. Barry,
Stephen L. Rogers,
Robert T. Fraley,
Leslie Brand
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.15.4776
Subject(s) - agrobacterium tumefaciens , cytokinin , gene , biology , plasmid , escherichia coli , locus (genetics) , genetics , enzyme , cloning (programming) , biosynthesis , ti plasmid , biochemistry , microbiology and biotechnology , transformation (genetics) , auxin , computer science , programming language
A small region of the Ti plasmid (the tmr locus), thought to be involved in phytohormone metabolism in Agrobacterium tumefaciens-transformed plant tissue, was cloned and expressed in Escherichia coli. By enzyme assay, the tmr locus was shown to encode isopentenyltransferase, an enzyme that catalyzes the first step in cytokinin biosynthesis.