Open AccessIdentification and primary sequence of an unspliced human urokinase poly(A)+ RNA.
Author(s) -
Pasquale Verde,
Maria Patrizia Stoppelli,
Patrizia Galeffi,
Pierpaolo Di Nocera,
Francesco Blasi
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.15.4727
Subject(s) - complementary dna , microbiology and biotechnology , biology , cdna library , untranslated region , rna , messenger rna , intron , coding region , nucleic acid sequence , oligonucleotide , urokinase , northern blot , gene , genetics
Human urokinase cDNA clones have been identified from a cDNA library prepared from total RNA of human fibroblasts transformed by simian virus 40 [Okayama, H. & Berg, P. (1983) Mol. Cell. Biol. 3, 280-289]. Synthetic oligonucleotides, corresponding to urokinase protein sequence, were used as probes. The cloned cDNA covers most of the coding sequence and the entire 3' untranslated region. The nucleotide sequence of one of the clones identifies this as a copy of a partially spliced polyadenylylated precursor to urokinase mRNA. The introns separate functionally different domains of the enzyme. Human urokinase mRNA has been identified by RNA blot and its size was estimated at 2500 nucleotides.