Open AccessPrimary structure of the Escherichia coli ribonucleoside diphosphate reductase operon.
Author(s) -
John G. Carlson,
James A. Fuchs,
Joachim Messing
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.14.4294
Subject(s) - operon , ribonucleotide reductase , open reading frame , escherichia coli , nucleotide , biology , gene , nucleic acid sequence , biochemistry , protein primary structure , structural gene , microbiology and biotechnology , genetics , peptide sequence , protein subunit
The nucleotide sequence of the Escherichia coli K-12 DNA comprising the operon for the structural genes of the subunits of ribonucleotide diphosphate reductase has been determined. The DNA sequenced maps at 48.5 minutes on the E. coli chromosome and includes a total length of 8557 nucleotides. An open reading frame between nucleotides 3506 and 5834, encoding a 776-amino acid polypeptide chain with a molecular weight of 87,532, has been identified as the nrdA gene. An open reading frame between nucleotides 6012 and 7139, encoding a 375-amino acid polypeptide with a molecular weight of 43,466, has been identified as the nrdB gene. The sequences reveal not only the primary structures for both subunits, but also some interesting aspects of potential regulatory sites.