Open AccessRecombination of transfected DNAs in vertebrate cells in culture.
Author(s) -
Pradip K. Bandyopadhyay,
Shinichi Watanabe,
Howard M. Temin
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.11.3476
Subject(s) - recombination , homologous recombination , transfection , biology , flp frt recombination , non allelic homologous recombination , homologous chromosome , genetic recombination , dna , microbiology and biotechnology , virus , cre lox recombination , site specific recombination , homology (biology) , in vitro recombination , genetics , virology , gene , complementary dna , recombinase , molecular cloning , transgene , genetically modified mouse
We studied the frequency of homologous and illegitimate recombinations between transfected noninfectious retroviral DNA molecules in chicken embryo fibroblasts. The frequency of recombination was determined by the formation of infectious virus and varied with the extent of homology between the DNA molecules at the site of recombination, but only when there were regions of homology surrounding this region. While homologous recombination led to the formation of wild-type virus, illegitimate recombination resulted in formation of infectious virus with alterations at the site of recombination. Apparent homologous recombination was also observed between transfected and chromosomal DNAs in D17 dog cells.