Open AccessReconstitution of a functional synaptosomal membrane possessing the protein constituents involved in acetylcholine translocation.
Author(s) -
Maurice Israël,
B. Lesbats,
Nicolas Morel,
R. Manaranche,
T. Gulik-Krzywicki,
Jean-Claude Dedieu
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.1.277
Subject(s) - acetylcholine , cytosol , membrane , biophysics , chemistry , membrane protein , calcium , acetylcholine receptor , biochemistry , microbiology and biotechnology , biology , pharmacology , receptor , enzyme , organic chemistry
Reconstitution of a functional presynaptic membrane possessing calcium-dependent acetylcholine release properties has been achieved. The proteoliposomal membrane obtained gains its acetylcholine-releasing capabilities from presynaptic membrane proteins. At the peak of acetylcholine release, intramembrane particles became more numerous in one of the proteoliposomal membrane faces. This phenomenon resembles the intramembrane particle rearrangements found in stimulated synaptosomes. No visible structures capable of releasing acetylcholine as a result of the calcium influx were found inside the proteoliposomes. This supports the view that the release of free cytosolic acetylcholine from stimulated nerve terminals can be directly attributed to presynaptic membrane proteins. These proteins were extracted in a functional form from the synaptosomal membrane.