Open AccessProtective immunization of the squirrel monkey against asexual blood stages of Plasmodium falciparum by use of parasite protein fractions.
Author(s) -
Philippe Dubois,
Jean-Pierre Dedet,
Thierry Fandeur,
Christian Roussilhon,
Moncef Jendoubi,
Serge Pauillac,
Odile Mercereau−Puijalon,
L Pereira Da Silva
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.1.229
Subject(s) - saimiri sciureus , squirrel monkey , biology , plasmodium falciparum , parasite hosting , antibody , virology , polyacrylamide gel electrophoresis , malaria , biochemistry , immunology , enzyme , neuroscience , world wide web , computer science
We had previously shown that two polypeptides of Plasmodium falciparum are preferentially recognized by antibodies of resistant squirrel monkeys Saimiri sciureus. Free parasites were isolated from synchronized cultures on human erythrocytes initially inoculated with infected Saimiri erythrocytes. Crude extracts were prepared from mature schizont stages and electrophoresed on preparative NaDodSO4/polyacrylamide gels. Two groups of five monkeys were immunized with protein fractions eluted from the 75- and 100-kilodalton regions of the gels. Strong protection against challenge by the homologous strain of P. falciparum was induced in both groups. Analysis of specific anti-malarial antibodies revealed a homogeneous response of all the animals against a few polypeptides of the mature parasite.