Open AccessTwo-dimensional 1H NMR study of the lambda operator site OL1: a sequential assignment strategy and its application.
Author(s) -
Michael A. Weiss,
Dinshaw J. Patel,
Robert Sauer,
Martin Karplus
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.1.130
Subject(s) - lambda , nucleic acid , operator (biology) , base pair , base (topology) , bacteriophage , chemistry , repressor , physics , stereochemistry , mathematics , dna , biochemistry , escherichia coli , mathematical analysis , quantum mechanics , transcription factor , gene
The solution structure and dynamics of the 17-base-pair synthetic operator site OL1, which is recognized by the cI and Cro repressors of bacteriophage lambda, is studied by two-dimensional NMR methods. A sequential assignment strategy for nucleic acids is proposed and illustrated by the assignments of the base and sugar protons of OL1.