Development of large granular lymphocytes with anomalous, nonspecific cytotoxicity in clones derived from Ly-2+ T cells. | Zendy

Ken Shortman | Zendy; Anne Wilson | Zendy; Roland Scollay | Zendy; W F Chen | Zendy

Open Access

Development of large granular lymphocytes with anomalous, nonspecific cytotoxicity in clones derived from Ly-2+ T cells.

Author(s) -

Ken Shortman,

Anne Wilson,

Roland Scollay,

W F Chen

Publication year - 1983

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.80.9.2728

Subject(s) - lysis , cytolysis , cytotoxicity , microbiology and biotechnology , concanavalin a , biology , cytotoxic t cell , clone (java method) , t lymphocyte , lymphocyte , cell , cell culture , chemistry , immunology , in vitro , immune system , biochemistry , dna , genetics

T cells cultured at limit dilution for 8 days in a concanavalin A-stimulated, filler-cell and growth factor-supported system produced cytolytic clones with high efficiency. These clones were not specific, lysing a wide range of targets, syngeneic and allogeneic, of tumor and normal cell origin. Lysis was a cell-mediated phenomenon but was not blocked by anti-Ly-2. One H-2-negative target was lysed, but one was resistant. Xenogeneic (human) tumor cells were not lysed. The cells in the clones were large, vacuolated, granular lymphocytes. They originated from single Ly-2+ responder cells and not from irradiated filler cells. Therefore, activated lymphocyte killers and other natural killer-like cells may be differentiated elements of the Ly-2+ T-cell lineage.

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