Open AccessEscherichia coli extract-catalyzed recombination in switch regions of mouse immunoglobulin genes.
Author(s) -
Takao Kataoka,
Shunichi Takeda,
Tasuku Honjo
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.9.2666
Subject(s) - recombination , escherichia coli , gene , biology , genetic recombination , lambda phage , genetics , flp frt recombination , microbiology and biotechnology , site specific recombination , immunoglobulin light chain , gene conversion , homologous recombination , antibody , bacteriophage , recombinase
We have shown that Escherichia coli extracts catalyze recombination between mouse immunoglobulin mu and alpha genes inserted separately in lambda phage vectors carrying different genetic markers. Most of the recombination sites in the inserts are located in the switch regions of the heavy chain genes, as previously found in the expressed genes of myeloma cells. The recombination took place at relatively high frequency (10(-4)). The recombinational system in E. coli or lambda phage seems to prefer short nucleotide sequences similar to those used in the class switch recombination.