Open AccessLinkage of beta 2-microglobulin and ly-m11 by molecular cloning and DNA-mediated gene transfer.
Author(s) -
David H. Margulies,
Jane R. Parnes,
Nancy Johnson,
J. G. Seidman
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.8.2328
Subject(s) - microbiology and biotechnology , biology , beta (programming language) , gene , beta 2 microglobulin , clone (java method) , genomic dna , thymidine kinase , cloning (programming) , genetics , virus , herpes simplex virus , computer science , immunology , programming language
beta 2-Microglobulin (beta 2m) is expressed on the cell surface after introduction of a beta 2mb (C57BL/6N) genomic clone into thymidine kinase-deficient mouse L cells by cotransformation using the calcium phosphate precipitate method. Stable transformant cell lines were identified that express the beta 2mb allele, as determined by reaction of the cells with appropriate monoclonal antibodies and by two-dimensional gel electrophoresis of endogenously labeled immunoprecipitates of cell extracts. These beta 2mb transformants now express ly-m11.2, as detected by an indirect radioimmunoassay. A plasmid subclone of the beta 2mb gene that contains an 8.4-kilobase insert, after introduction into mouse L cells, similarly directs the synthesis of both the beta 2mb and the ly-m11.2 antigens. Thus, the beta 2mb and ly-m11.2 determinants most likely represent sites on the same protein structure.