Functional changes of intermediate filaments in fibroblastic cells revealed by a monoclonal antibody. | Zendy

Renato Dulbecco | Zendy; R Allen | Zendy; Seiji Okada | Zendy; M Bowman | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Functional changes of intermediate filaments in fibroblastic cells revealed by a monoclonal antibody.

Author(s) -

Renato Dulbecco,

R Allen,

Seiji Okada,

M Bowman

Publication year - 1983

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.80.7.1915

Subject(s) - intermediate filament , vimentin , monoclonal antibody , polyclonal antibodies , microbiology and biotechnology , monoclonal , immunofluorescence , indirect immunofluorescence , antibody , biology , chemistry , cell culture , cell , biophysics , cytoskeleton , immunohistochemistry , biochemistry , immunology , genetics

We describe reversible changes of intermediate filaments of fibroblastic cells associated with changes in the functional state of the cells. The changes are revealed by comparing the immunofluorescence patterns given by a monoclonal antibody and a polyclonal serum, both recognizing vimentin. The state of the filaments depends on culture density; this effect cannot be attributed to the nutritional state of the cells, their growth rate, or substances released into the medium. It seems to depend mainly on the aggregation of filaments during strong cell movements. The possible significance of these findings for the functional role of intermediate filaments is discussed.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research