Open AccessHomologous upstream sequences near Epstein-Barr virus promoters.
Author(s) -
Paul J. Farrell,
Prescott L. Deininger,
Alan T. Bankier,
B. G. Barrell
Publication year - 1983
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.6.1565
Subject(s) - promoter , biology , transcription (linguistics) , microbiology and biotechnology , dna , rna , virus , ecori , base pair , virology , genetics , gene , gene expression , plasmid , philosophy , linguistics
The sequence of the 17,166-base-pair EcoRI C fragment of Epstein-Barr virus DNA, cell line B95-8, was determined. In vitro transcription was used to identify three RNA polymerase II promoters within this fragment of the virus. Cytoplasmic poly(A)+ RNAs starting at these points were demonstrated in B95-8 cells induced into virus production with 12-O-tetradecanoylphorbol 13-acetate. Uninduced B95-8 cells contained much less of these RNAs. The upstream sequences near the three promoters show striking homologies that may be involved in transcriptional control.
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