Open AccessStructure of a Bacillus subtilis bacteriophage SPO1 gene encoding RNA polymerase sigma factor.
Author(s) -
Maria C. Costanzo,
J. Pero
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.5.1236
Subject(s) - sigma factor , rna polymerase , nucleic acid sequence , biology , gene , promoter , genetics , bacillus subtilis , microbiology and biotechnology , homology (biology) , bacteriophage , polymerase , rna , escherichia coli , gene expression , bacteria
Gene 28 of Bacillus subtilis bacteriophage SPO1 codes for a regulatory protein, a sigma factor known as sigma gp28, that binds to the bacterial core RNA polymerase to direct the recognition of phage middle gene promoters. middle promoters exhibit distinctive and conserved nucleotide sequences in two regions centered about 10 and 35 base pairs upstream from the start point of mRNA synthesis. Here we report the cloning of gene 28 and its complete nucleotide sequence. We infer that sigma gp28 is a 25,707-dalton protein of 220 amino acids. Neither the nucleotide sequence of gene 28 nor the inferred amino acid sequence of sigma gp28 exhibits extensive homology to the gene or protein sequence of Escherichia coli sigma factor.