Open AccessCharacterization and partial nucleotide sequence of endogenous type C retrovirus segments in human chromosomal DNA.
Author(s) -
Roy Repaske,
Raymond O'Neill,
Paul E. Steele,
Malcolm A. Martin
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.3.678
Subject(s) - biology , dna , nucleic acid sequence , microbiology and biotechnology , retrovirus , restriction enzyme , murine leukemia virus , nucleic acid thermodynamics , endogenous retrovirus , nucleic acid , nucleotide , restriction map , peptide sequence , virology , genetics , gene , genome , base sequence
Twenty-six different murine leukemia virus (MuLV)-related clones have been isolated from a human DNA library and characterized by restriction enzyme mapping and reciprocal nucleic acid hybridization reactions. The sequence of approximately 2,600 nucleotides, spanning more than 4.0 kilobases, of one of the MuLV-related cloned human DNAs was also determined. The deduced amino acid sequence permitted the alignment of this prototype cloned human DNA segment with the p12 gag, p30 gag, p10 gag, and pol regions of Moloney MuLV. A majority of the endogenous type C retrovirus-related segments present in human DNA are approximately 6.0 kilobases in size and appear to contain a deletion of env sequences.