Open AccessDifferent exon-intron organization at the 5' part of a mouse class I gene is used to generate a novel H-2Kd-related mRNA.
Author(s) -
JeanLouis Lalanne,
Madeleine Cochet,
Anne-Marie Kummer,
Gabriel Gachelin,
Philippe Kourilsky
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.24.7561
Subject(s) - intron , exon , gene , biology , open reading frame , rna splicing , genetics , nucleic acid sequence , complementary dna , microbiology and biotechnology , alternative splicing , messenger rna , sequence (biology) , peptide sequence , rna
A cDNA library constructed from liver mRNA of DBA/2 (H-2d) mice has been screened with H-2-specific probes. The nucleotide sequence of one clone (pH-2d-24) indicates that it derives from an H-2 gene with an unexpected exon-intron organization. Nucleotide sequence comparisons suggest that two distinct mRNAs are produced from a single H-2Kd gene by a mechanism involving the use of alternative splicing sites in its 5' region. pH-2d-24 carries an open reading frame encoding a thus-far-undescribed polypeptide product identical to an H-2Kd-molecule, except for the NH2-terminal half of the first domain.