Open AccessAmplified C lambda and c-abl genes are on the same marker chromosome in K562 leukemia cells.
Author(s) -
Jules R. Selden,
B S Emanuel,
E Wang,
Linda A. Cannizzaro,
Antônio Palumbo,
Jan Erikson,
Peter C. Nowell̀,
Giovanni Rovera,
Carlo M. Croce
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.23.7289
Subject(s) - chronic myelogenous leukemia , k562 cells , abl , microbiology and biotechnology , philadelphia chromosome , biology , chromosome , leukemia , centromere , gene , chromosome 22 , genetics , chromosomal translocation , tyrosine kinase , signal transduction
The human leukemia cell line K562, derived from a patient with Philadelphia chromosome-positive chronic myelogenous leukemia, contains amplified c-abl oncogenes and unrearranged C lambda genes. Using in situ hybridization techniques, we have determined that the amplified c-abl and C lambda DNA sequences of K562 cells are both located on the same abnormal acrocentric marker chromosome, which may represent an altered Philadelphia chromosome.