Open AccessGenetic recombination of bacteriophage lambda DNAs in Xenopus oocytes.
Author(s) -
Dana Carroll
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.22.6902
Subject(s) - xenopus , bacteriophage , recombination , biology , genetic recombination , genetics , dna , oocyte , recombinant dna , microbiology and biotechnology , cre lox recombination , in vitro recombination , escherichia coli , molecular cloning , embryo , gene , transgene , genetically modified mouse , peptide sequence
Pairs of genetically marked bacteriophage lambda DNAs have been injected into Xenopus laevis oocyte nuclei. After suitable incubation, DNA was recovered and packaged into phage particles in vitro. When these were plated onto a selective host, phage recombinant for parental markers were observed. Recombination was dependent on both parents being present in the same oocyte nucleus and was roughly proportional to the physical separation of the markers. Thus, the oocytes appear to contain the machinery necessary for performing typical genetic recombination. This system offers a great deal of scope and flexibility for future studies of recombination mechanisms at the molecular level in vertebrates.