Open AccessMultiple quantum two-dimensional 1H--15N nuclear magnetic resonance spectroscopy: chemical shift correlation maps for exchangeable imino protons of Escherichia coli tRNAMetf in water.
Author(s) -
Richard H. Griffey,
C. Dale Poulter,
Ad Bax,
B. L. HAWKINS,
Ziro Yamaizumi,
Susumu Nishimura
Publication year - 1983
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.19.5895
Subject(s) - chemical shift , nuclear magnetic resonance spectroscopy , spectroscopy , protonation , nuclear magnetic resonance , chemistry , escherichia coli , two dimensional nuclear magnetic resonance spectroscopy , dispersion (optics) , resonance (particle physics) , proton , physics , atomic physics , biochemistry , nuclear physics , optics , organic chemistry , quantum mechanics , ion , gene
A procedure based on multiple quantum two-dimensional nuclear magnetic resonance spectroscopy is described for generation of 1H--15N chemical shift correlation maps. The method is used to obtain 15N chemical shifts for the exchangeable imino protons in 1H--15N units of site-specifically labeled Escherichia coli tRNAMetf in water. The high sensitivity and excellent chemical shift dispersion of the multiple quantum two-dimensional technique make it ideally suited for studying protonated nitrogens by NMR.
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