Open AccessTwo-dimensional electrophoresis of plasma polypeptides reveals "high" heterozygosity indices.
Author(s) -
Barnett B. Rosenblum,
James V. Neel,
Samir Hanash
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.16.5002
Subject(s) - loss of heterozygosity , polyacrylamide gel electrophoresis , silver stain , microbiology and biotechnology , staining , gel electrophoresis , biology , electrophoresis , two dimensional gel electrophoresis , genetic variants , chemistry , genetics , genotype , biochemistry , allele , gene , proteomics , enzyme
A series of 62 plasma samples have been examined for genetic variation by the technique of two-dimensional polyacrylamide gel electrophoresis followed by silver-staining of the gels. Twenty polypeptides chosen without respect to variability were considered suitable for scoring. Of the total of 1,240 polypeptides, 29 could not be scored unambiguously. Seventy-five of the remaining 1,211 exhibited the combination of a normal and a variant polypeptide. All variants were present in either the father or the mother of the subject. This index of heterozygosity (6.2 +/- 0.7%) is substantially higher than those reported by others in similar studies of human fibroblasts, lymphocytes, kidney, or brain cells.