Open AccessRETRACTED: A precise termination site in the mouse beta major-globin transcription unit.
Author(s) -
M. Salditt-Georgieff,
James Darnell
Publication year - 1983
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.15.4694
Subject(s) - transcription (linguistics) , microbiology and biotechnology , biology , gene , rna , dna , nuclease , rna polymerase , polymerase , globin , rna polymerase ii , genetics , promoter , gene expression , linguistics , philosophy
Nascent labeled RNA from induced, globin-producing mouse erythroleukemia cells was hybridized to cloned regions of the beta major-globin gene. Transcription ceases about 1,000 bases downstream from the poly(A) site as indicated by protection from nuclease digestion of a discrete-sized RNA fragment that it shorter than the protecting cloned DNA fragment. This defines an apparently unique termination site for a protein-coding gene that is transcribed by RNA polymerase II.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom