Open AccessA precise termination site in the mouse beta major-globin transcription unit.
Author(s) -
M Salditt-Georgieff,
Jr Je Darnell
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.15.4694
Subject(s) - transcription (linguistics) , microbiology and biotechnology , biology , gene , rna , dna , nuclease , rna polymerase , polymerase , globin , rna polymerase ii , genetics , promoter , gene expression , philosophy , linguistics
Nascent labeled RNA from induced, globin-producing mouse erythroleukemia cells was hybridized to cloned regions of the beta major-globin gene. Transcription ceases about 1,000 bases downstream from the poly(A) site as indicated by protection from nuclease digestion of a discrete-sized RNA fragment that it shorter than the protecting cloned DNA fragment. This defines an apparently unique termination site for a protein-coding gene that is transcribed by RNA polymerase II.