Open AccessLocalization of prostaglandin D2 binding protein and NADP-linked 15-hydroxyprostaglandin D2 dehydrogenase in the Purkinje cells of miniature pig cerebellum.
Author(s) -
Yasuyoshi Watanabe,
Akira Yamashita,
Hidekado Tokumoto,
Osamu Hayaishi
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.14.4542
Subject(s) - cerebellum , purkinje cell , cerebellar cortex , dehydrogenase , prostaglandin , granular layer , immunohistochemistry , biology , prostaglandin d2 , biochemistry , enzyme , chemistry , microbiology and biotechnology , endocrinology , immunology
High-affinity binding sites specific for prostaglandin D2 were visualized by the autoradiographical technique assisted by computerized image processing and color coding. In the cerebellum of miniature pig, high density of the binding was observed in the cortex, mainly in the Purkinje cell layer. Immunohistochemical studies on the cellular localization of NADP-linked 15-hydroxyprostaglandin D2 dehydrogenase were performed with the same cerebellum and revealed the presence of this enzyme also in the Purkinje cells. These findings imply that prostaglandin D2 binds to the Purkinje cells and, after its action, is degraded in situ by the specific degradative enzyme.