Open AccessCell-type-specific gene expression and acatalasemic peroxisomes in a null Cat2 catalase mutant of maize
Author(s) -
Athanasios Tsaftaris,
Artemios M. Bosabalidis,
John G. Scandalios
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.14.4455
Subject(s) - mutant , catalase , peroxisome , biology , vascular bundle , gene , microbiology and biotechnology , gene expression , wild type , gene product , biochemistry , botany , enzyme
Cell separation studies in conjunction with immunocytochemical studies indicate that mesophyll cells and bundle-sheath cells, the dimorphic photosynthetic cell types in the leaves of the C4 plantZea mays , differ in their catalase composition. In particular, catalase-2, the product of theCat2 gene, is found primarily in the bundle-sheath cells, whereas catalase-3, the product of theCat3 gene, is found primarily in the mesophyll cells. Electron microscopic observations reveal that bundle-sheath cells of A16, a mutant line lacking expression of theCat2 gene in all tissues examined, contain numerous peroxisomes, but they are acatalasemic as determined by staining with 3,3′-diaminobenzidine. The significance of this mutant in physiological studies is discussed.