Open AccessPermanent cell line expressing human factor VIII-related antigen established by hybridization.
Author(s) -
CoraJean S. Edgell,
C McDonald,
John B. Graham
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.12.3734
Subject(s) - antigen , biology , cell culture , microbiology and biotechnology , umbilical vein , population , progenitor cell , immunofluorescence , endothelial stem cell , stem cell , antibody , immunology , genetics , in vitro , medicine , environmental health
A permanent human cell line, EA . hy 926, has been established that expresses at least one highly differentiated function of vascular endothelium, factor VIII-related antigen. This line was derived by fusing human umbilical vein endothelial cells with the permanent human cell line A549. Hybrid cells that survived in selective medium had more chromosomes than either progenitor cell type and included a marker chromosome from the A549 line. Factor VIII-related antigen can be identified intracellularly in the hybrids by immunofluorescence and accumulates in the culture fluid. Expression of factor VIII-related antigen by these hybrid cells has been maintained for more than 100 cumulative population doublings, including more than 50 passages and three cloning steps. This is evidence that EA . hy 926 represents a permanent line.