Open AccessIdentification of a structural gene for a RNA polymerase II polypeptide in Drosophila melanogaster and mammalian species.
Author(s) -
C J Ingles,
Joseph R. Biggs,
Jacqueline Wong,
J R Weeks,
Arno L. Greenleaf
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.11.3396
Subject(s) - biology , microbiology and biotechnology , polymerase , drosophila melanogaster , rna polymerase i , dna , rna polymerase iii , gene , rna , dna polymerase ii , dna polymerase , rna polymerase ii , chinese hamster ovary cell , rna dependent rna polymerase , genetics , gene expression , promoter , reverse transcriptase , cell culture
Using subclones representing 14 kilobase pairs (kb) of DNA from the Drosophila melanogaster RNA polymerase II (EC 2.7.7.6) X-linked genetic locus, RpII, we have identified four poly(A)+ RNA transcripts in adult flies. The DNA encoding only one of these, a 7-kb transcript, cross-hybridized to mammalian DNA. DNA from alpha-amanitin-resistant (AmaR) Chinese hamster ovary (CHO) and human cells was used to transform the temperature-sensitive (TS) RNA polymerase II Syrian hamster mutant TsAF8. The acquisition of the TS+ AmaR RNA polymerase II phenotype was accompanied by the appearance of donor-DNA-specific restriction fragments that cross-hybridize to the D. melanogaster 7-kb transcript DNA. This D. melanogaster DNA and the related DNA detected in mammalian species must therefore be the structural gene for a RNA polymerase II polypeptide.