Open AccessCloning of the gene for C protein, a low molecular weight spore-specific protein from Bacillus megaterium.
Author(s) -
Everardo Curiel-Quesada,
Barbara Setlow,
Peter Setlow
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.11.3250
Subject(s) - bacillus megaterium , biology , gene , transcription (linguistics) , microbiology and biotechnology , molecular cloning , dna , promoter , escherichia coli , cloning (programming) , gene expression , genetics , bacteria , linguistics , philosophy , computer science , programming language
The structural gene for C protein, a low molecular weight spore-specific protein from Bacillus megaterium, has been cloned in Escherichia coli. Expression of the C-protein gene in E. coli requires an external transcription promoter and prevention of termination of transcription prior to transcription of all or part of the sequence coding for the C protein. The gene for the C protein is within a 5-kilobase DNA fragment, but this fragment does not code for either of the other two major low molecular weight spore proteins, suggesting that the structural genes for these proteins are not tightly linked.