Metal and sulfur composition of iron-molybdenum cofactor of nitrogenase. | Zendy

Mark Nelson | Zendy; Mark A. Levy | Zendy; W H Orme-Johnson | Zendy

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Metal and sulfur composition of iron-molybdenum cofactor of nitrogenase.

Author(s) -

Mark Nelson,

Mark A. Levy,

W H Orme-Johnson

Publication year - 1983

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.80.1.147

Subject(s) - molybdenum , nitrogenase , cofactor , sulfur , chemistry , metal , methionine , cysteine , inorganic chemistry , iron–sulfur cluster , molybdenum cofactor , biochemistry , enzyme , nitrogen , organic chemistry , amino acid , nitrogen fixation

The sulfur content of N-methylformamide solutions of cofactor from Clostridium pasteurianum nitrogenase has been determined to be 11.9 (+/- 0.9) mol per mol of molybdenum. This number was determined radiochemically, using iron-molybdenum cofactor isolated from molybdenum-iron protein from bacteria grown on 35SO4. A total of 3.2 (+/- 0.2) mol of sulfur per mol of molybdenum was found to be present in cysteine and methionine, probably arising from contaminating proteins not intrinsic to the cofactor. Combined with accumulated evidence that is discussed, these results lead to an updated stoichiometry of MoFe6S8 or 9, not MoFe6S4 as previously thought, for this cluster.

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