Open AccesscDNA cloning and induction of the alcohol dehydrogenase gene ( Adh1 ) of maize
Author(s) -
W. L. Gerlach,
A. Pryor,
E. S. Dennis,
Robert J. Ferl,
Martin M. Sachs,
W. James Peacock
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.9.2981
Subject(s) - complementary dna , biology , messenger rna , alcohol dehydrogenase , microbiology and biotechnology , biochemistry , gene , nucleic acid sequence , molecular cloning , locus (genetics) , enzyme , genetics
cDNA clones ofAdh1 , one of two genes encoding alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1) in the maize genome, have been isolated. They were derived from mRNA extracted from anaerobically treated roots of maize seedlings. Identification was initially made on the basis of molecular weight and electrophoretic properties of thein vitro polypeptide obtained in hybridization-release-translation experiments. The identification was confirmed by antibody precipitation and by the use of maize stocks having different genetic constitutions at theAdh1 locus. The sequence of the longest cDNA segment, ≈900 base pairs, was determined and appears to code for 168 COOH-terminal amino acids and to have a 3′ nontranslated region of 364 base pairs. Reverse Southern hybridizations established that two differentAdh1-S stocks produce a mRNA of 1,650 nucleotides, whereas an additional mRNA of 1,750 nucleotides is produced in threeAdh1-F stocks. A 50-fold increase inAdh1 mRNA level occurs during anaerobiosis, reaching a maximum at 5 hr. Return to aerobic conditions indicates a half-life of more than 18 hr for the anaerobically inducedAdh1 mRNA.