Open AccessCharacterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.
Author(s) -
Dinah S. Singer,
R. Daniel CameriniOtero,
M. L. Satz,
Barbara A. Osborne,
David H. Sachs,
Stuart Rudikoff
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.5.1403
Subject(s) - biology , major histocompatibility complex , microbiology and biotechnology , heterologous , antigen , genomic dna , complementary dna , clone (java method) , histocompatibility , cdna library , gene , human leukocyte antigen , genetics
A porcine genomic clone encoding a major histocompatibility, (MHC) antigen was isolated by direct screening of a swine genomic library with a heterologous human MHC cDNA probe. Mouse L cells transformed with DNA from the clone stably express swine MHC antigen. Pig alloantisera specifically lyse transformant but not control cell lines in a complement-mediated cytotoxicity assay. Direct immunoprecipitation of radiolabeled cellular protein from transformed lines by pig alloantiserum results in the coprecipitation of swine MHC heavy chain and mouse beta 2-microglobulin, demonstrating the association of heterologous subunits of MHC antigens.