Open AccessSite-directed mutagenesis of a tRNA gene: base alterations in the coding region affect transcription.
Author(s) -
M S Ciampi,
Douglas A. Melton,
Riccardo Cortese
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.5.1388
Subject(s) - gene , biology , genetics , transcription (linguistics) , xenopus , mutant , base pair , point mutation , microbiology and biotechnology , philosophy , linguistics
Point mutations have been introduced in vitro in a cloned nematode tRNAPro gene. Four different mutant clones altered in the DNA that codes for tRNAPro have been isolated. Studies on the expression of the mutant genes by microinjection into Xenopus oocyte nuclei reveal that their activities as transcription templates are reduced. The results show that DNA sequences that code for the extra arm and the stem of the T-psi-C-G arm of the tRNAPro have an important role in the initiation of tRNA gene transcription.