Open AccessReplication and phenotypic expression of control and scleroderma human fibroblasts: responses to growth factors.
Author(s) -
E. Carwile LeRoy,
S. Mercurio,
Glenn K. Sherer
Publication year - 1982
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.4.1286
Subject(s) - fibroblast , growth factor , platelet derived growth factor , epidermal growth factor , fibroblast growth factor , biology , scleroderma (fungus) , transforming growth factor , human skin , microbiology and biotechnology , immunology , cancer research , endocrinology , in vitro , platelet derived growth factor receptor , cell culture , genetics , receptor , inoculation
To explore the mechanism of increased collagen synthesis by scleroderma skin fibroblasts in vitro, control and scleroderma fibroblasts were compared in confluent monolayer cultures growth-arrested by serum deprivation; responses to optimal mitogenic doses of platelet-derived growth factor, fibroblast growth factor, epidermal growth factor and nerve growth factor were compared. Platelet-derived growth factor had a selective mitogenic effect on control skin fibroblasts not observed with scleroderma skin fibroblasts. None of the factors studied had a selective effect on collagen synthesis independent of cell replication; scleroderma and control fibroblasts responded similarly. Therefore, the growth factors studied may not be involved in generating the activated scleroderma fibroblast directly; platelet-derived growth factor may play an indirect role in fibroblast replication in human fibrotic disorders.U
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