Open AccessTwo-dimensional rhodopsin crystals from disk membranes of frog retinal rod outer segments.
Author(s) -
Joseph M. Corless,
Darrell R. McCaslin,
Burton L. Scott
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.4.1116
Subject(s) - rhodopsin , orthorhombic crystal system , membrane , crystallography , retinal , chemistry , biophysics , biology , crystal structure , biochemistry
Two-dimensional crystals of rhodopsin have been prepared from purified frog disk membranes by using the detergent Tween 80. The space group of the orthorhombic crystals is p22121; the unit cell dimensions are 47 X 151 A. Projection maps of negatively stained preparations have been calculated to a resolution of approximately 22 A. The rhodopsin molecules are associated as dimers that appear to be slightly sigmoidal and are 20-25 A in width and 70-80 A in length.