Open AccessChromosomal localization of human leukocyte, fibroblast, and immune interferon genes by means of in situ hybridization
Author(s) -
Jeffrey M. Trent,
Sharon Olson,
Richard M. Lawn
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.24.7809
Subject(s) - microbiology and biotechnology , biology , gene , interferon , chromosome , metaphase , plasmid , in situ hybridization , complementary dna , chromosome 15 , immune system , alpha interferon , recombinant dna , interferon gamma , fibroblast , chromosome 12 , genetics , cell culture , gene expression
Recombinant plasmids containing cDNA inserts from human leukocyte interferon (IFN-α), fibroblast interferon (IFN-β), or immune interferon (INF-γ) genes were radiolabeled and hybridizedin situ to human metaphase chromosome preparations. The results localized the human IFN-α and IFN-β genes to the short arm of chromosome 9(p21→pter) and localized the IFN-γ gene to the long arm of chromosome 12(q24.1).