Open AccessMutant immunoglobulin genes have repetitive DNA elements inserted into their intervening sequences.
Author(s) -
Robert G. Hawley,
Marc J. Shulman,
Helios Murialdo,
D. M. Gibson,
Nobumichi Hozumi
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.23.7425
Subject(s) - immunoglobulin light chain , kappa , mutant , gene , biology , dna , microbiology and biotechnology , genetics , immunoglobulin gene , antibody , philosophy , linguistics
The kappa light chain genes from two mutant hybridoma cell lines defective in kappa light chain synthesis were isolated and compared to the wild-type kappa light chain gene. In each case, the mutant kappa light chain genes were found to contain repetitive DNA elements in their intervening sequences that were not present in the intervening sequences of the wild-type kappa light chain gene. These elements were found to be related to the genes of intracisternal A particles. These results suggest that the decreased production of kappa light chain in the mutant cell lines is due to the presence of the intracisternal A particle-related genes.