Open AccessSequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter.
Author(s) -
René Hen,
Paolo Sassone–Corsi,
Jeffry L. Corden,
MariePierre Gaub,
Pierre Chambon
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.23.7132
Subject(s) - in vivo , in vitro , biology , transcription (linguistics) , upstream (networking) , upstream activating sequence , promoter , transcription factor , microbiology and biotechnology , serotype , computational biology , virology , gene , genetics , gene expression , computer science , computer network , philosophy , linguistics
We show that sequences located upstream from the T-A-T-A box, between positions -97 and -34, are necessary for efficient in vivo transcription from the adenovirus serotype 2 major late promoter. The effect of these upstream sequences was also investigated in vitro using a whole cell or an S100 extract and circular or linear templates. With the whole cell extract, the in vivo effect of the upstream sequences was reproduced in vitro. With the S100 extract, some effect of the upstream sequences was observed with circular, but not with linear, templates.