Purification and properties of a rat liver protein that specifically inhibits the proliferation of nonmalignant epithelial cells from rat liver. | Zendy

James B. McMahon | Zendy; James G. Farrelly | Zendy; P. Thomas Iype | Zendy

Open Access

Purification and properties of a rat liver protein that specifically inhibits the proliferation of nonmalignant epithelial cells from rat liver.

Author(s) -

James B. McMahon,

James G. Farrelly,

P. Thomas Iype

Publication year - 1982

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.79.2.456

Subject(s) - isoelectric point , isoelectric focusing , cell growth , microbiology and biotechnology , ultrafiltration (renal) , polyacrylamide gel electrophoresis , sodium dodecyl sulfate , gel electrophoresis , biochemistry , chemistry , cell culture , biology , chromatography , enzyme , genetics

An inhibitor of cell proliferation was purified from rat liver by alcohol precipitation, ultrafiltration, and DEAE-cellulose chromatography. The hepatic proliferation inhibitor was shown to be pure by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, analytical isoelectric focusing, and high-performance liquid chromatography. The hepatic proliferation inhibitor was found to have a molecular weight of 26,000 and an isoelectric point of 4.65. This protein inhibited the proliferation of nonmalignant rat liver cells in culture, and removal of the protein reversed the inhibition produced by low doses. It exerted no effect on the proliferation of malignant rat liver cells.

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