Coated vesicles from human placenta carry ferritin, transferrin, and immunoglobulin G. | Zendy

Barbara M. F. Pearse | Zendy

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Coated vesicles from human placenta carry ferritin, transferrin, and immunoglobulin G.

Author(s) -

Barbara M. F. Pearse

Publication year - 1982

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.79.2.451

Subject(s) - vesicle , ferritin , transferrin , chemistry , isotonic , antibody , immunoglobulin g , biochemistry , placenta , membrane , biology , immunology , fetus , medicine , pregnancy , genetics

Coated vesicles prepared by using isotonic gradients retain their contents. Ferritin, transferrin, and immunoglobulin G have been detected in human placental coated vesicles. Triton X-100 extraction of crude coated vesicles removes contaminating uncoated membranes, leaving coated particles and their associated proteins apparently intact; this provides an efficient means of preparing large quantities of coated vesicle proteins. The clathrin cages surround "cores" of structural protein containing polypeptides of 100,000 and 50,000 molecular weight.

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