Open AccessOrientation of cohesive end site cos determines the active orientation of chi sequence in stimulating recA . recBC-mediated recombination in phage lambda lytic infections.
Author(s) -
Ichizo Kobayashi,
Helios Murialdo,
Jean M. Crasemann,
Mary M. Stahl,
Franklin W. Stahl
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.19.5981
Subject(s) - recombination , lytic cycle , orientation (vector space) , bacteriophage , lambda phage , dna , recbcd , physics , lambda , homologous recombination , lysogenic cycle , escherichia coli , biology , microbiology and biotechnology , genetics , sequence (biology) , dna repair , geometry , optics , gene , virus , mathematics
Sequence chi, 5'G-C-T-G-G-T-G-G, locally enhances homologous recombination by recA and recBC proteins of Escherichia coli. Previous work showed that, in phage lambda, chi is more active in one orientation (leftward) than in the other (rightward). Inverting cos, the sequence for the mature DNA ends of lambda, reverses this orientation dependence: the rightward chi becomes fully active, and the leftward chi becomes relatively inactive. We surmise that chi action in phage lambda is coupled with DNA packaging or injection.