Open AccessMismatch repair, gene conversion, and crossing-over in two recombination-defective mutants of Drosophila melanogaster.
Author(s) -
Adelaide T. C. Carpenter
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.19.5961
Subject(s) - heteroduplex , recombination , genetics , gene conversion , biology , mutant , drosophila melanogaster , chromosomal crossover , mitotic crossover , flp frt recombination , genetic recombination , homologous recombination , ectopic recombination , dna , gene , locus (genetics) , allele , dna repair
Recombination-defective mutants at two loci that are known to decrease drastically the frequency of meiotic crossing-over do not decrease the frequency of gene conversion at the rosy locus. mei-9 mutant alleles produce frequent postmeiotic segregants manifested as mosaic progeny whereas controls and mei-218 mutants produce none. It is concluded that (i) recombination in Drosophila involves a biparental DNA intermediate and (ii) correction of heteroduplex DNA or recognition of biparental DNA or both is necessary, but not sufficient, for this intermediate to result in crossing-over of flanking markers. It is therefore likely, at least in Drosophila, that the isomerization step in Meselson-Radding type molecular models of recombination is under genetic control.